Analysis of the mechanisms of interaction between the soluble receptor Tas2r1 and lymphocyte transcription factors in various types of bronchial asthma
ORIGINAL PAPERS
Abstract
Introduction. We continue to study the role of ectopic taste receptors in the pathogenesis of bronchial asthma (BA). Previously, an analysis of the association of plasma levels of one of the 25 known subtypes of these receptors Tas2R1 with clinical and functional characteristics of different variants of BA was conducted. The purpose of this article is an additional analysis of possible mechanisms of interaction in BA transcription factors and negative regulators of gene transcription: STAT6, STAT4, T-bet, SOCS1, SOCS3. Materials and methods. The study included 10 practically healthy individuals, 36 patients with allergic bronchial asthma (ABA) and 20 with non-allergic bronchial asthma (NABA). The level of Tas2R1 in the blood serum was determined by the enzyme immunoassay. For the study, lymphocytes from the peripheral blood of healthy individuals and patients with BA, isolated on a density gradient, were used. The methods for determining transcription factors included immunoblotting and RT-PCR. Results. A direct relationship was found between the expression of serum Tas2R1 and an increase in the expression of the transcription factor STAT6, as well as its active form p STAT6 under the action of interleukin 4. In addition, a direct relationship was found between serum Tas2R1 and the factors STAT4 and T-bet. A positive relationship between the Tas2R1 receptor determined in serum and the expression of SOCS1 and SOCS3 molecules can be due to the influence of both STAT6 and STAT4. A negative relationship was found between the expression of serum Tas2R1 and the expression of m RNA of the transcription factor PAX-5 in ABA. The role of the only specific transcription factor for Tas2R receptors — SREBP-2, found in enteroendocrine cells of the STC-1 line of the gastrointestinal tract of mice is discussed. Conclusion. Further study of the putative interactions of the Tas2R1 receptor and lymphocyte transcription factors will allow us to determine in more detail the contribution of ectopic bitter taste receptors to the pathogenesis of BA at the level of the micronetwork of transcription factors.
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